Asteroids have been shown to produce a great diversity of unique secondary metabolites, including a wide variety of saponins. Elucidation of saponins from asteroids has shown that each species produces a unique repertoire of saponin structures. During my PhD thesis research, I have utilized the Crown-of-Thorns Starfish to evaluate existing and novel saponin purification and identification methods. The outcomes have demonstrated the differences between traditional liquid-liquid partitioning, column chromatography and membrane diffusion. Additionally, this research has identified saponins present in the conditioned CoTS water, which may lead to the development of a CoTS-specific biosensor techniques.